講座名稱：基于單分子定位的超分辨光學(xué)顯微技術(shù)及應(yīng)用

講座人：李依明

講座時(shí)間：10月15日15:50

講座地點(diǎn)：北校區(qū)西大樓Ⅰ區(qū)-210

講座人介紹：

李依明，2009年于上海交通大學(xué)獲生物醫(yī)學(xué)工程專業(yè)工程學(xué)士學(xué)位，2010年于德國海德堡大學(xué)獲醫(yī)學(xué)物理碩士學(xué)位，2015年師從于Uli Nienhaus教授，于德國卡爾斯魯厄理工學(xué)院獲得生物物理博士學(xué)位。2016-2019年在歐洲分子生物實(shí)驗(yàn)室海德堡總部做博士后，期間前往耶魯大學(xué)擔(dān)任訪問學(xué)者。目前為南方科技大學(xué)生物醫(yī)學(xué)工程系終身助理教授。以第一作者在Nature Methods、ACS Nano，以及Optics Letters等國際知名科學(xué)期刊上發(fā)表多篇論文。其開發(fā)的軟件獲領(lǐng)域內(nèi)權(quán)威挑戰(zhàn)賽（SMLM挑戰(zhàn)賽，2016年）第一名。研究興趣包括前沿三維超分辨率成像技術(shù)及其生物應(yīng)用的發(fā)展。

講座內(nèi)容：

Interferometric photoactivated localization microscopy (iPALM, 4Pi-SMS) uses multiphase interferometry to localize single molecules and currently achieves the highest axial resolution of all 3D superresolution approaches. In theory, 3D sub-10 nm resolution can be achieved with only 250 photons collected in each objective for an individual molecule. However, the resolution achievable with the current image analysis workflow is substantially worse than the theoretical limit. Here, we developed an experimental PSF fitting method for the interference 4Pi-PSF. As the interference phase is not fixed with respect to the shape of the PSF, we developed a new 4Pi-PSF model, which decouples the phase term from the shape of the PSF. Using a spline-interpolated experimental PSF model and by fitting all 3 or 4 phase images globally, we showed on simulated data that we can achieve the theoretical limit of 3D resolution, the Cramér-Rao lower bound (CRLB), also for 4Pi microscope.

主辦單位：物理與光電工程學(xué)院